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semidry transfer apparatus  (Bio-Rad)


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    Structured Review

    Bio-Rad semidry transfer apparatus
    Semidry Transfer Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 3992 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trans+blot+sd+semidry+transfer+apparatus/Trans-Blot+SD/pm40282462-78-21-29
    Average 99 stars, based on 3992 article reviews
    semidry transfer apparatus - by Bioz Stars, 2026-10
    99/100 stars

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    SDS Page:

    Article Title: Distinct roles of centriole distal appendage proteins in ciliary assembly and disassembly
    Article Snippet: Protein concentration was determined using the BCA assay (Pierce). .. Equal amounts of protein were loaded onto SDS-PAGE gels, and transferred to polyvinylidene difluoride (PVDF) membrane (Millipore) using a Trans-Blot SD semidry transfer apparatus (Bio-Rad). .. Membranes were blocked for 1 h in TBST containing 5% skim milk or BSA, followed by overnight at 4 °C with the following primary antibodies: mouse anti-Aurora A (MyBiosource, MBS212609, 1:1000), mouse anti-β-actin (Santa Cruz, sc-47778, 1:10000).

    Article Title: Distinct roles of centriole distal appendage proteins in ciliary assembly and disassembly.
    Article Snippet: Protein concentration was determined using the BCA assay (Pierce). .. Equal amounts of protein were loaded onto SDS-PAGE gels, and transferred to polyvinylidene difluoride (PVDF) membrane (Millipore) using a Trans-Blot SD semidry transfer apparatus (Bio-Rad). .. Membranes were blocked for 1 h in TBST containing 5% skim milk or BSA, followed by overnight at 4 °C with the following primary antibodies: mouse anti-Aurora A (MyBiosource, MBS212609, 1:1000), mouse anti-β-actin (Santa Cruz, sc-47778, 1:10000).

    Article Title: A cooperative PNPase-Hfq-RNA carrier complex facilitates bacterial riboregulation
    Article Snippet: .. Western blotting analysis was performed by fractionating the samples reserved for protein analysis on a 4% stacking 10% resolving SDS-PAGE gel in 1 × Tris-glycine SDS buffer at 120 V. Fractionated protein was then transferred to a 0.45 μm PVDF membrane (Thermo Scientific) at 15 V for 30 min using Trans-Blot SD semidry transfer apparatus (Bio-rad) following manufacturer’s guidelines. ..

    Membrane:

    Article Title: Distinct roles of centriole distal appendage proteins in ciliary assembly and disassembly
    Article Snippet: Protein concentration was determined using the BCA assay (Pierce). .. Equal amounts of protein were loaded onto SDS-PAGE gels, and transferred to polyvinylidene difluoride (PVDF) membrane (Millipore) using a Trans-Blot SD semidry transfer apparatus (Bio-Rad). .. Membranes were blocked for 1 h in TBST containing 5% skim milk or BSA, followed by overnight at 4 °C with the following primary antibodies: mouse anti-Aurora A (MyBiosource, MBS212609, 1:1000), mouse anti-β-actin (Santa Cruz, sc-47778, 1:10000).

    Article Title: The five homologous CiaR-controlled Ccn sRNAs of Streptococcus pneumoniae modulate Zn-resistance.
    Article Snippet: .. Briefly, 3 μg of isolated RNA was fractionated on 10% polyacrylamide gels containing 7% urea by electrophoresis at 55 V and subsequently, transferred to a Zeta-probe membrane (Bio-Rad) using a Trans-Blot SD semidry transfer apparatus (Bio-rad) at 4 mA per cm2 with a maximum of 400 mA for 50 min. RNA was then UV-crosslinked to the membrane with a Spectroline UV crosslinker with the "optimal crosslink" setting. .. 5’-Biotinylated probes were hybridized to the membrane overnight at 42 ̊C in ULTRAhyb (Ambion) hybridization buffer.

    Article Title: The five homologous CiaR-controlled Ccn sRNAs of Streptococcus pneumoniae modulate Zn-resistance
    Article Snippet: .. Briefly, 3 μg of isolated RNA was fractionated on 10% polyacrylamide gels containing 7% urea by electrophoresis at 55 V and subsequently, transferred to a Zeta-probe membrane (Bio-Rad) using a Trans-Blot SD semidry transfer apparatus (Bio-rad) at 4 mA per cm 2 with a maximum of 400 mA for 50 min. RNA was then UV-crosslinked to the membrane with a Spectroline UV crosslinker with the "optimal crosslink" setting. .. 5’-Biotinylated probes were hybridized to the membrane overnight at 42 ° C in ULTRAhyb (Ambion) hybridization buffer.

    Article Title: Distinct roles of centriole distal appendage proteins in ciliary assembly and disassembly.
    Article Snippet: Protein concentration was determined using the BCA assay (Pierce). .. Equal amounts of protein were loaded onto SDS-PAGE gels, and transferred to polyvinylidene difluoride (PVDF) membrane (Millipore) using a Trans-Blot SD semidry transfer apparatus (Bio-Rad). .. Membranes were blocked for 1 h in TBST containing 5% skim milk or BSA, followed by overnight at 4 °C with the following primary antibodies: mouse anti-Aurora A (MyBiosource, MBS212609, 1:1000), mouse anti-β-actin (Santa Cruz, sc-47778, 1:10000).

    Article Title: A cooperative PNPase-Hfq-RNA carrier complex facilitates bacterial riboregulation
    Article Snippet: .. Western blotting analysis was performed by fractionating the samples reserved for protein analysis on a 4% stacking 10% resolving SDS-PAGE gel in 1 × Tris-glycine SDS buffer at 120 V. Fractionated protein was then transferred to a 0.45 μm PVDF membrane (Thermo Scientific) at 15 V for 30 min using Trans-Blot SD semidry transfer apparatus (Bio-rad) following manufacturer’s guidelines. ..

    Article Title: Pivotal Roles for Ribonucleases in Streptococcus pneumoniae Pathogenesis
    Article Snippet: RNA concentration was measured using a NanoDrop 2000 (Thermo Fisher Scientific). .. Two micrograms of each RNA sample was loaded on 10% polyacrylamide gels containing 7 M urea or loaded onto 10% Criterion TBE-urea precast gels (Bio-Rad) and electrophoresed at 85 V. RNA samples were transferred to a Zeta-Probe GT membrane (Bio-Rad) using a Trans-Blot SD semidry transfer apparatus (Bio-Rad) following the manufacturer’s guidelines. .. Transferred RNA was UV cross-linked and hybridized overnight with 100 ng/ml of 5′ biotinylated DNA probe ( ) in Ultrahyb (Ambion) hybridization buffer at 42°C.

    Article Title: The five homologous CiaR-controlled Ccn sRNAs of Streptococcus pneumoniae modulate Zn-resistance
    Article Snippet: .. Briefly, 3 μg of isolated RNA was fractionated on 10% polyacrylamide gels containing 7% urea by electrophoresis at 55 V and subsequently, transferred to a Zeta-probe membrane (Bio-Rad) using a Trans-Blot SD semidry transfer apparatus (Bio-rad) at 4 mA per cm 2 with a maximum of 400 mA for 50 min. RNA was then UV-crosslinked to the membrane with a Spectroline UV crosslinker with the “optimal crosslink” setting. .. 5’-Biotinylated probes were hybridized to the membrane overnight at 42°C in ULTRAhyb (Ambion) hybridization buffer.

    Article Title: A cooperative PNPase-Hfq-RNA carrier complex facilitates bacterial riboregulation
    Article Snippet: .. RNA was then transferred to a Zeta-probe membrane (Bio-Rad) using the Trans-Blot SD semidry transfer apparatus (Bio-Rad) per manufacturer’s instructions. .. RNA was UV-crosslinked to the membrane and probed for each given RNA using a 5′ biotinylated DNA oligo of complementary sequence in ULTRAhyb (Ambion) hybridization buffer at 42°C.

    Isolation:

    Article Title: The five homologous CiaR-controlled Ccn sRNAs of Streptococcus pneumoniae modulate Zn-resistance.
    Article Snippet: .. Briefly, 3 μg of isolated RNA was fractionated on 10% polyacrylamide gels containing 7% urea by electrophoresis at 55 V and subsequently, transferred to a Zeta-probe membrane (Bio-Rad) using a Trans-Blot SD semidry transfer apparatus (Bio-rad) at 4 mA per cm2 with a maximum of 400 mA for 50 min. RNA was then UV-crosslinked to the membrane with a Spectroline UV crosslinker with the "optimal crosslink" setting. .. 5’-Biotinylated probes were hybridized to the membrane overnight at 42 ̊C in ULTRAhyb (Ambion) hybridization buffer.

    Article Title: The five homologous CiaR-controlled Ccn sRNAs of Streptococcus pneumoniae modulate Zn-resistance
    Article Snippet: .. Briefly, 3 μg of isolated RNA was fractionated on 10% polyacrylamide gels containing 7% urea by electrophoresis at 55 V and subsequently, transferred to a Zeta-probe membrane (Bio-Rad) using a Trans-Blot SD semidry transfer apparatus (Bio-rad) at 4 mA per cm 2 with a maximum of 400 mA for 50 min. RNA was then UV-crosslinked to the membrane with a Spectroline UV crosslinker with the "optimal crosslink" setting. .. 5’-Biotinylated probes were hybridized to the membrane overnight at 42 ° C in ULTRAhyb (Ambion) hybridization buffer.

    Article Title: The five homologous CiaR-controlled Ccn sRNAs of Streptococcus pneumoniae modulate Zn-resistance
    Article Snippet: .. Briefly, 3 μg of isolated RNA was fractionated on 10% polyacrylamide gels containing 7% urea by electrophoresis at 55 V and subsequently, transferred to a Zeta-probe membrane (Bio-Rad) using a Trans-Blot SD semidry transfer apparatus (Bio-rad) at 4 mA per cm 2 with a maximum of 400 mA for 50 min. RNA was then UV-crosslinked to the membrane with a Spectroline UV crosslinker with the “optimal crosslink” setting. .. 5’-Biotinylated probes were hybridized to the membrane overnight at 42°C in ULTRAhyb (Ambion) hybridization buffer.

    Electrophoresis:

    Article Title: The five homologous CiaR-controlled Ccn sRNAs of Streptococcus pneumoniae modulate Zn-resistance.
    Article Snippet: .. Briefly, 3 μg of isolated RNA was fractionated on 10% polyacrylamide gels containing 7% urea by electrophoresis at 55 V and subsequently, transferred to a Zeta-probe membrane (Bio-Rad) using a Trans-Blot SD semidry transfer apparatus (Bio-rad) at 4 mA per cm2 with a maximum of 400 mA for 50 min. RNA was then UV-crosslinked to the membrane with a Spectroline UV crosslinker with the "optimal crosslink" setting. .. 5’-Biotinylated probes were hybridized to the membrane overnight at 42 ̊C in ULTRAhyb (Ambion) hybridization buffer.

    Article Title: The five homologous CiaR-controlled Ccn sRNAs of Streptococcus pneumoniae modulate Zn-resistance
    Article Snippet: .. Briefly, 3 μg of isolated RNA was fractionated on 10% polyacrylamide gels containing 7% urea by electrophoresis at 55 V and subsequently, transferred to a Zeta-probe membrane (Bio-Rad) using a Trans-Blot SD semidry transfer apparatus (Bio-rad) at 4 mA per cm 2 with a maximum of 400 mA for 50 min. RNA was then UV-crosslinked to the membrane with a Spectroline UV crosslinker with the "optimal crosslink" setting. .. 5’-Biotinylated probes were hybridized to the membrane overnight at 42 ° C in ULTRAhyb (Ambion) hybridization buffer.

    Article Title: The five homologous CiaR-controlled Ccn sRNAs of Streptococcus pneumoniae modulate Zn-resistance
    Article Snippet: .. Briefly, 3 μg of isolated RNA was fractionated on 10% polyacrylamide gels containing 7% urea by electrophoresis at 55 V and subsequently, transferred to a Zeta-probe membrane (Bio-Rad) using a Trans-Blot SD semidry transfer apparatus (Bio-rad) at 4 mA per cm 2 with a maximum of 400 mA for 50 min. RNA was then UV-crosslinked to the membrane with a Spectroline UV crosslinker with the “optimal crosslink” setting. .. 5’-Biotinylated probes were hybridized to the membrane overnight at 42°C in ULTRAhyb (Ambion) hybridization buffer.

    Western Blot:

    Article Title: A cooperative PNPase-Hfq-RNA carrier complex facilitates bacterial riboregulation
    Article Snippet: .. Western blotting analysis was performed by fractionating the samples reserved for protein analysis on a 4% stacking 10% resolving SDS-PAGE gel in 1 × Tris-glycine SDS buffer at 120 V. Fractionated protein was then transferred to a 0.45 μm PVDF membrane (Thermo Scientific) at 15 V for 30 min using Trans-Blot SD semidry transfer apparatus (Bio-rad) following manufacturer’s guidelines. ..



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